TY - JOUR AU - Maulina, Syarifah Aulia AU - Soulissa, Abdul Gani AU - Widyarman, Armelia Sari PY - 2023/01/09 TI - Antibiofilm Effect of Rambutan Leaf Extract (Nephelium lappaceum L.) on Selected Periodontal Pathogens JF - Journal of Indonesian Dental Association; Vol 5 No 2 (2022): OctoberDO - 10.32793/jida.v5i2.924 KW - N2 -   Introduction: In Indonesia, periodontal disease is one of the dental and oral diseases with the highest prevalence. Bacteria in subgingival plaque can cause periodontal disease, such as Porphyromonas gingivalis and Fusobacterium nucleatum . Herbal medicine has potential as an alternative in the treatment of periodontal disease, for example, rambutan leaves ( Nephelium lappaceum L.) are known to have antibacterial properties because they contain flavonoids, tannins, and saponins. Objective: To determine the effects of rambutan leaf extract ( Nephelium lappaceum L.) on development of Porphyromonas gingivalis and Fusobacterium nucleatum biofilms. Methods: This study was carried out using the biofilm assay method with crystal violet staining and rambutan leaf extract ( Nephelium lappaceum L.) with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, and 3.125% were used as the test material. A microplate reader with a wavelength of 490 nm was used to measure the biofilm density of Porpyromonas gingivalis ATCC 33277 and Fusobacterium nucleatum ATCC 25586. The data obtained were then analyzed statistically using one-way analysis of variance (ANOVA), with a significance level of p<0.05. Results: The most effective concentration of rambutan leaf extract for inhibiting biofilm development at 24 hours incubation was a 100% concentration with an average optical density (OD) of 0.147 against Porphyromonas gingivalis and a 100% concentration with an average OD of 0.077 against Fusobacterium nucleatum . Conclusion: Rambutan leaf extract ( Nephelium lappaceum L.) is capable of inhibiting the development of Porphyromonas gingivalis and Fusobacterium nucleatum biofilms, respectively. UR - http://jurnal.pdgi.or.id/index.php/jida/article/view/924