The Effect of Presto Cooker as an Alternative Sterilizer Device for Standard Dental Equipment

  • Ariadna Adisattya Djais Department of Oral Biology, Faculty of Dentistry, University of Indonesia, Indonesia
  • Citra Fragrantia Theodorea Department of Oral Biology, Faculty of Dentistry, University of Indonesia, Indonesia

Abstract

 
Introduction: In the suburb area of Indonesia, autoclaves as a sterilizer could not been used optimally due to inadequate electrical capacity. An alternative sterilizer such as a pressure cooker (presto) have been choosen because it has same principle as an autoclave, but it doesn’t required the electrical supply. Nevertheless, the procedure of presto in dentistry remain unclear.
 
Objective: To obtain a standard procedure by using presto for dental instrument.
 
Methods: The effect of presto was observed on aerobic (S. aureus ATCC 25923T), facultative anaerobes (S. mutans ATCC 25175T), anaerobes (P. gingivalis ATCC 33277T) and yeasts (C. albicans ATCC 10231T) which are exposed to the dental mirror. Each dental mirror (triplo) was dipped for 3 minutes on media containing bacteria (106 bacteria/ mL). Furthermore, the dental mirrors were cooked at presto (MAXIM, 7 L, Indonesia) which contained 500 mL of water, for 15, 30 and 45 minutes. Bacterial growth analysis were observed visually and microscopically after Gram staining.
 
Results: In the S. aureus ATCC 25923T and C. albicans ATCC 10231T groups, up to 30 minutes the color of the media showed cloudy but remained clear when sterilized for 45 minutes. Likewise, these groups showed appereance of bacterial growth for 15-30 minutes but didn’t appear to grow in 45 minutes. While in the S. mutans ATCC 25175T and P. gingivalis ATCC 33277T groups, up to 15 minutes the color of the media showed cloudy but remained clear after being sterilized for 30 minutes. In addition, these groups showed appereance of bacterial growth for 15 minutes but absence in 30-45 minutes.
 
Conclusion: Presto can be used as one of alternative equipment to sterilize dental instrument, effectively. The optimal killing time of bacteria and yeast was 45 minutes.

References

Bargale S, Patel N, Dave B, Poonacha KS, Ardeshana A, Mehta D. Assessment of sterilization technique practices at dental clinics of Vadodara, Gujarat India. Sch J Dent Sci. 2016;3(9):240-246.
Alder VG, Brown AM, Gillespie WA. Disinfection of heat-sensitive material by low-temperature steam and formaldehyde. J Clin Pathol. 1966;19(1):83-89.
N Vishal Gupta, Shukshith KS. Qualification of autoclave. Int J Pharm Tech Res. 2016; 9(4):220-226.
Madigan MT, Martinko JM, Brock TD. Brock Biology of Microorganisms. New Jersey: Pearson Prentice Hall; 2006.
Baird RM, Hodges NA, Denyer SP. 2000. Handbook of microbiological quality control: pharmaceutical science. USA: CRC Press; 2000.
DD Date, Tated RG. Pressure cooker lid-literature review. International Journal of Advance Research Ideas and Innovations in Technology (IJARIIT). 2018;4(3):2388-96.
Everall PH, Morris CA, Yarnell R. Sterilization in the laboratory autoclave using direct air displacement by steam. J Clin Pathol. 1978;31(2):144-147.
Marin LFC, Arciniegas GE, and Vivas MC. Characterization of Staphylococcus aureus isolates that colonize medical students in a hospital on the city of Cali, Columbia. Int J Microbiol. 2015;2015:10358489.
Abulkasim HM, GS Shukla, HK Bajaj and Masih H. Antimicrobial resistance of Staphylococcus aureus among healthy and adult students. Int J Pharm Sci Res. 2017; 8(12):5247-5251.
Cheon K, Moser SA, Wienner HW, Whiddon J, Momeni SS, Ruby JD, et al. Characteristics of Streptococcus mutans genotypes and dental caries in children. Eur J Oral Sci. 2013; 121(301):148-155.
Mounika S, Jagannathan N, Murali. Association of Streptococcus mutans and Streptococcus sanguis in act of dental caries. J Pharm Sci Res. 2015;7(9):764-766.
How KY, Song KP, Chan KG. Phorphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. Front Microbiol. 2016;7:53.
Lof M, Janus MM, Krom BP. Metabolic interaction between bacteria and fungi in comensal oral biofilms. J Fungi. 2017;3(3):40.
Ghasempour M, Sefidgar SA, Eyzadian H, Gharakhani S. Prevalence of Candida albicans in dental plague and caries lesion of early childhood caries (ECC) according to sampling site. Caspian J Intern Med. 2011;2(4):304-308.
Adhikari R, Pant ND, Neupane S, Neupane N, Bhattarai R, Bhattas, et al. Detection of methicilin resistant Staphylococcus aureus and determination of minimum inhibitory concentration of vancomycin for Staphylococcus aureus isolated from pus /wound swab samples of patients attending a tertiary care hospital in Kathmandu Nepal. Can J Infect Dis Med Microbiol. 2017;2017(2191532):1-6.
Bargale S, Patel N, Dave B, Poonacha KS, Ardeshana A, Mehta D. Assessment of sterilization technique practices at dental clinics of Vadodara, Gujarat India. Sch J Dent Sci. 2016;3(9):240-246.
Alder VG, Brown AM, Gillespie WA. Disinfection of heat-sensitive material by low-temperature steam and formaldehyde. J Clin Pathol. 1966;19(1):83-89.
N Vishal Gupta, Shukshith KS. Qualification of autoclave. Int J Pharm Tech Res. 2016; 9(4):220-226.
Madigan MT, Martinko JM, Brock TD. Brock Biology of Microorganisms. New Jersey: Pearson Prentice Hall; 2006.
Baird RM, Hodges NA, Denyer SP. 2000. Handbook of microbiological quality control: pharmaceutical science. USA: CRC Press; 2000.
DD Date, Tated RG. Pressure cooker lid-literature review. International Journal of Advance Research Ideas and Innovations in Technology (IJARIIT). 2018;4(3):2388-96.
Everall PH, Morris CA, Yarnell R. Sterilization in the laboratory autoclave using direct air displacement by steam. J Clin Pathol. 1978;31(2):144-147.
Marin LFC, Arciniegas GE, and Vivas MC. Characterization of Staphylococcus aureus isolates that colonize medical students in a hospital on the city of Cali, Columbia. Int J Microbiol. 2015;2015:10358489.
Abulkasim HM, GS Shukla, HK Bajaj and Masih H. Antimicrobial resistance of Staphylococcus aureus among healthy and adult students. Int J Pharm Sci Res. 2017; 8(12):5247-5251.
Cheon K, Moser SA, Wienner HW, Whiddon J, Momeni SS, Ruby JD, et al. Characteristics of Streptococcus mutans genotypes and dental caries in children. Eur J Oral Sci. 2013; 121(301):148-155.
Mounika S, Jagannathan N, Murali. Association of Streptococcus mutans and Streptococcus sanguis in act of dental caries. J Pharm Sci Res. 2015;7(9):764-766.
How KY, Song KP, Chan KG. Phorphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. Front Microbiol. 2016;7:53.
Lof M, Janus MM, Krom BP. Metabolic interaction between bacteria and fungi in comensal oral biofilms. J Fungi. 2017;3(3):40.
Ghasempour M, Sefidgar SA, Eyzadian H, Gharakhani S. Prevalence of Candida albicans in dental plague and caries lesion of early childhood caries (ECC) according to sampling site. Caspian J Intern Med. 2011;2(4):304-308.
Adhikari R, Pant ND, Neupane S, Neupane N, Bhattarai R, Bhattas, et al. Detection of methicilin resistant Staphylococcus aureus and determination of minimum inhibitory concentration of vancomycin for Staphylococcus aureus isolated from pus /wound swab samples of patients attending a tertiary care hospital in Kathmandu Nepal. Can J Infect Dis Med Microbiol. 2017;2017(2191532):1-6.
Bargale S, Patel N, Dave B, Poonacha KS, Ardeshana A, Mehta D. Assessment of sterilization technique practices at dental clinics of Vadodara, Gujarat India. Sch J Dent Sci. 2016;3(9):240-246.
Alder VG, Brown AM, Gillespie WA. Disinfection of heat-sensitive material by low-temperature steam and formaldehyde. J Clin Pathol. 1966;19(1):83-89.
N Vishal Gupta, Shukshith KS. Qualification of autoclave. Int J Pharm Tech Res. 2016; 9(4):220-226.
Madigan MT, Martinko JM, Brock TD. Brock Biology of Microorganisms. New Jersey: Pearson Prentice Hall; 2006.
Baird RM, Hodges NA, Denyer SP. 2000. Handbook of microbiological quality control: pharmaceutical science. USA: CRC Press; 2000.
DD Date, Tated RG. Pressure cooker lid-literature review. International Journal of Advance Research Ideas and Innovations in Technology (IJARIIT). 2018;4(3):2388-96.
Everall PH, Morris CA, Yarnell R. Sterilization in the laboratory autoclave using direct air displacement by steam. J Clin Pathol. 1978;31(2):144-147.
Marin LFC, Arciniegas GE, and Vivas MC. Characterization of Staphylococcus aureus isolates that colonize medical students in a hospital on the city of Cali, Columbia. Int J Microbiol. 2015;2015:10358489.
Abulkasim HM, GS Shukla, HK Bajaj and Masih H. Antimicrobial resistance of Staphylococcus aureus among healthy and adult students. Int J Pharm Sci Res. 2017; 8(12):5247-5251.
Cheon K, Moser SA, Wienner HW, Whiddon J, Momeni SS, Ruby JD, et al. Characteristics of Streptococcus mutans genotypes and dental caries in children. Eur J Oral Sci. 2013; 121(301):148-155.
Mounika S, Jagannathan N, Murali. Association of Streptococcus mutans and Streptococcus sanguis in act of dental caries. J Pharm Sci Res. 2015;7(9):764-766.
How KY, Song KP, Chan KG. Phorphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. Front Microbiol. 2016;7:53.
Lof M, Janus MM, Krom BP. Metabolic interaction between bacteria and fungi in comensal oral biofilms. J Fungi. 2017;3(3):40.
Ghasempour M, Sefidgar SA, Eyzadian H, Gharakhani S. Prevalence of Candida albicans in dental plague and caries lesion of early childhood caries (ECC) according to sampling site. Caspian J Intern Med. 2011;2(4):304-308.
Adhikari R, Pant ND, Neupane S, Neupane N, Bhattarai R, Bhattas, et al. Detection of methicilin resistant Staphylococcus aureus and determination of minimum inhibitory concentration of vancomycin for Staphylococcus aureus isolated from pus /wound swab samples of patients attending a tertiary care hospital in Kathmandu Nepal. Can J Infect Dis Med Microbiol. 2017;2017(2191532):1-6.
Rafiei M, Kiani F, Sayehmiri F, Sayehmiri K, Sheiki A, Azodi MZ. Study of Phorphyromonas gingivalis in periodontal disease: a systematic review and meta-analysis. Med J Islam Repub Iran. 2017;31: 62.
How KY, Song KP, and Chan KG. Porphyromonas gingivalis: An overview of periodontopathic pathogen below the gum line. Front Microbiol. 2016; 7: 53.
Published
2019-04-30
How to Cite
DJAIS, Ariadna Adisattya; THEODOREA, Citra Fragrantia. The Effect of Presto Cooker as an Alternative Sterilizer Device for Standard Dental Equipment. Journal of Indonesian Dental Association, [S.l.], v. 1, n. 2, p. 41-47, apr. 2019. ISSN 2621-6175. Available at: <http://jurnal.pdgi.or.id/index.php/jida/article/view/356>. Date accessed: 19 june 2019. doi: https://doi.org/10.32793/jida.v1i2.356.
Section
Research Article