Overview of the Total Bacteria and Number of Streptococcus mutans in the Saliva of Children with High Caries Activity
Abstract
Introduction: Microorganisms play a crucial role in the early stage of caries. Streptococcus mutans produces lactic acid, which enhances the risk of caries. Polymerase chain reaction (PCR) is a molecular biology technique used in the amplification and replication of DNA. PCR exhibits high specificity and a rapid detection time, as well as differentiation between microorganisms. Based on a previous study, there is a significant correlation between S. mutans in saliva and the prevalence of caries. Objectives: To provide an overview of the total bacteria and number of S. mutans in the saliva of children aged between 5 and 10 years at the Department of Pediatric, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia. Methods: Saliva sample from children (n = 28, aged 5-10 years old) was collected from healthy children. 2-3 mL of a saliva samples were collected and cultured by using TYS media for total bacterial count. The number of S. mutans in the saliva of these children was counted by real-time PCR methods by using a fluorescence chemical compound green SYBR using 16S rRNA gene specific primers for S. mutans. Results: The average number of total bacterial colony count in saliva was 9.965 ± 2.078 log10 CFU/mL, and the average number of S. mutans in the saliva of children was 8.303 ± 2.351 log10 CFU/mL. Conclusion: The number of S. mutans in the saliva of children in Department of Pediatric, Faculty of Dentistry, Trisakti University was categorized is in the high-risk.References
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Indonesia. Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar (RISKESDAS) 2018. Jakarta: Badan Penelitian dan Pengembangan Kesehatan. 2018. pp.81-202.
Rosdiana N, Nasution AI. Gambaran daya hambat minyak kelapa murni dan minyak kayu putih dalam menghambat pertumbuhan Streptococcus mutans. J Syiah Kuala Dent Soc. 2016;1(1):43-50.
Pradopo S. The colony number of Streptococcus mutans and Lactobacillus in saliva of dental caries and free caries children. Maj Ked Gigi (Dent J). 2008;41:53-55.
Chokshi A, Mahesh P, Sharada P, Chokshi K, Anupriya S, Ashwini B. A correlative study of the levels of salivary Streptococcus mutans , lactobacilli and Actinomyces with dental caries experience in subjects with mixed and permanent dentition. J Oral Maxillofac Pathol. 2016;20(1):25-8.
Sylvania DA, Gultom FP, Bachtiar BM. Korelasi kuantitas Streptococcus mutans pada plak lidah dan saliva dengan risiko karies tinggi – Corelation between quantity of Streptococcus mutans in tongue plaque and saliva and high risk caries. J FKG UI. 2014.
Acevedo A, Ray M, Socorro M, Rojas-Sánchez F. Frequency and distribution of Mutans Streptococci in dental plaque from caries-free and caries-affected Venezuelan children. Acta Odontol. 2009;22(1):15-20.
Alhamda S. Status kebersihan gigi dan mulut dengan status karies gigi (kajian pada murid kelompok umur 12 tahun di Sekolah Dasar Negeri Kota Bukittinggi). Berita Kedokteran Masyarakat. 2011;27(2):108-15.
Rotimi VO, Salako NO, Divia M, Asfour L, Kononen E. Prevalence of periodontal bacteria in saliva of Kuwait children at different age group. J Infect Public Health. 2010;3:76-82.
Widyarman AS, Hartono V, Marjani LI, Irawan D, Luthfi L, Bachtiar BM. Lactobacillus reuteri containing probiotic lozenges consumption reduces Streptococcus mutans, Streptococcus sobrinus, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans in orthodontic patients. J Int Dent Res. 2018;11(2):628-633.
Yoshizawa JM. Salivary biomarkers: toward future clinical and diagnostic utilities. 2013;26:781-791. Natamiharja L, Margaret. Peran orangtua terhadap pemeliharaan kesehatan gigi dan mulut anak kelas II SD Medan. Dentika Dent J. 16:163.
RoSanz AMG, Nieto BAG, Nieto EG. Dental health: relationship between dental health and food consumption. Nutr Hosp. 2013;28(Suppl4):64-71.
Forssten SD, Björklund M, Ouwehand AC. Streptococcus mutans, caries and simulation models. Nutrients. 2010;2(3):290-298.
RoSanz AMG, Nieto BAG, Nieto EG. Dental health: relationship between dental health and food consumption. Nutr Hosp. 2013;28(Suppl4):64-71.
Alphianti LT, Mei A. Jigsaw versus Conventional Method towards Children’s Attitude and Knowledge of Dental and Oral Health. 2019;2(2):69-74.
Forssten SD, Björklund M, Ouwehand AC. Streptococcus mutans, caries and simulation models. Nutrients. 2010;2(3):290-298.
Published
2020-02-20
How to Cite
HARDINI YANIS, Natasya Putri; PUTRIANY AGUSTIN, Tri.
Overview of the Total Bacteria and Number of Streptococcus mutans in the Saliva of Children with High Caries Activity.
Journal of Indonesian Dental Association, [S.l.], v. 3, n. 1, p. 1-5, feb. 2020.
ISSN 2621-6175.
Available at: <http://jurnal.pdgi.or.id/index.php/jida/article/view/403>. Date accessed: 22 nov. 2024.
doi: https://doi.org/10.32793/jida.v3i1.403.
Issue
Section
Research Article
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
