Mahkota Dewa (God’s Crown) Fruit Extract Inhibits the Formation of Periodontal Pathogen Biofilms in vitro

Diajeng Celia Radita; Armelia Sari Widyarman

doi:10.32793/jida.v2i2.404

Diajeng Celia Radita Undergraduate Student, Faculty of Dentistry, Trisakti University
Armelia Sari Widyarman

DOI: https://doi.org/10.32793/jida.v2i2.404

Abstract

Introduction: Mahkota dewa (Phaleria macrocarpa) is an Indonesian fruit that contains antibacterial compounds, such as flavonoids, saponins, and tannins; it has been used as an alternative treatment for controlling infection. Objectives: This study aimed to examine the effect of mahkota dewa fruit extract on the formation of Porphyromonas gingivalis (P. gingivalis), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), and Treponema denticola (T. denticola) biofilms in vitro. Methods: God’s crown fruit was extracted using the maceration technique, and then diluted into different concentrations (25%, 12.5%, 6.25%, 3.125%, and 1.56%) using phosphate buffered saline (PBS). P. gingivalis ATCC-33277, A. actinomycetemcomitans ATCC-29522, or T. denticola ATCC-35405 were cultured in brain heart infusion (BHI) broth, 24h (anaerobic-condition), and then each type of bacteria (108CFU/mL) was distributed into a 96-well microplate to form a biofilm. Subsequently, the fruit extracts were distributed into the biofilm-containing well plates and incubated for 1h, 6h, and 24h. A biofilm without the fruit extract and chlorhexidine-gluconate (0.2%) was used as the negative and positive control, respectively. Crystal violet (0.5%w/v) was used to determine the density of the remaining biofilm using a microplate spectrophotometer (600 nm). Data were statistically analyzed using one-way ANOVA, and p <0.05 was set as the level of significance. Results: The mahkota dewa fruit extracts significantly inhibited the formation of a biofilm for all three bacterial strains at all concentrations and for each incubation time (p <0.05) based on optical density (OD)±SD. The best concentration of fruit extract to inhibit biofilm formation was 25% for P. gingivalis (OD=0.19±0.06), 12.5% for A. actinomycetemcomitans (OD=0.14 ± 0.16), and 25% for T. denticola (OD=1.17±0.19) in comparison to the biofilm mass of the negative control, which was 1.67±0.06, 1.17±0.34, 2.66±0.38 for P. gingivalis, A. actinomycetemcomitans, and T. denticola, respectively. Conclusion: Based on these results, mahkota dewa fruit extract can inhibit the formation of biofilm on P. gingivalis, A. actinomycetemcomitans, and T. denticola, and it may potentially be used to prevent the infection associated with periodontal disease.

Author Biography

Armelia Sari Widyarman

References

1. Palombo EA. Traditional medicinal plant extracts and natural products with activity against oral bacteria: Potential application in the prevention and treatment of oral diseases. eCAM. 2011;8(1):1–11.
2. Kementrian Kesehatan Republik Indonesia. InfoDATIN (Pusat Data dan Informasi Kementrian Kesehatan RI). Situasi Kesehatan Gigi dan Mulut. Jakarta, Indonesia; 2014. pp 1.
3. Bahekar AA, Singh S, Saha S, Molnar J, Arora R. The prevalence and incidence of coronary heart disease is significantly increased in periodontitis: A meta-analysis. Am Hear J. 2007;154(5):830–7.
4. Kim TS, Schenk A, Lungeanu D, Reitmeir P, Eickholz P. Nonsurgical and surgical periodontal therapy in single-rooted teeth. Clin Oral Investig. 2007;11(4):391–9.
5. Kothari S, Gnanaranjan G, Kothiyal P. Periodontal chip: An adjunct to conventional surgical treatment. Int J Drug Res Tech. 2012;2(6):411-421.
6. Armitage GC. Comparison of the microbiological features of chronic and aggressive periodontitis. Periodontol 2000. 2010;53(1):70–88.
7. Thomas JG, Nakaishi LA. Managing the complexity of a dynamic biofilm. J Am Dent Assoc. 2006;137:10–15.
8. Hojo K, Nagaoka S, Ohshima T, Maeda N. Bacterial interactions in dental biofilm development. J Dent Res. 2011;88(11):982-90.
9. Dias DA, Urban S, Roessner U. A historical overview of natural products in drug discovery. Metabolites. 2012;2:303-336.
10. Manian R, Anusuya N, Siddhuraju P, Manian S. The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficusbengalensis L. and Ficusracemosa L. Food Chem. 2008;107(3):1000–7.
11. Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E. Flavonoid analyses and antimicrobial activity of various parts of Phaleriamacrocarpa (Scheff.) Boerl fruit. Int J Mol Sci. 2011;12(6):3422–31.
12. Hendra R, Ahmad S, Oskoueian E, Sukari A, Shukor MY. Antioxidant, anti-inflammatory and cytotoxicity of Phaleriamacrocarpa (Boerl.) Scheff fruit. BMC Complement Altern Med. 2011;11(1):110.
13. Naharsari ND, Wahyudi F. Mahkota dewa dan manfaatnya. Bekasi: Ganeca Exact; 2007. p. 8.
14. Lay MM, Karsani SA, Mohajer S, Abd Malek SN. Phytochemical constituents, nutritional values, phenolics, flavonols, flavonoids, antioxidant and cytotoxicity studies on Phaleria macrocarpa (Scheff.) Boerl fruits. BMC Complement Altern Med. 2014;14(1):152.
15. Altaf R, Asmawi MZ Bin, Dewa A, Sadikun A, Umar MI. Phytochemistry and medicinal properties of Phaleria macrocarpa (Scheff.) Boerl. extracts. Pharmacogn Rev. 2013;7(13):73–80.
16. Shamsudin H, Yunus B, Uddin S. Bioactive compounds and advanced processing technology: Phaleria macrocarpa (sheff.) Boerl, a review. J Chem Technol Biotechnol. 2015;90(6):981–91.
17. Liliany D, Widyarman AS, Erfan E, Sudiono J, Djamil MS. Enzymatic activity of bromelain isolated from pineapple (Ananas comosus) hump and its antibacterial effect on Enterococcus faecalis. Sci Dent J. 2018;2(2):39-50.
18. Widyarman AS, Widjaja SB, Idrus E. Strawberry extract’s effects on Enterococcus faecalis and Porphyromonas gingivalis biofilms in vitro. Sci Dent J. 2017;1(1):1-5.
19. Susilawati, Matsjeh S, Pranowo HD, Anwar C. Two isophalerin compounds from ethyl acetate of leave and fruit of mahkotadewa (Phaleria macrocarpa (Scheff.) Boerl.) and its antibacterial activity. Indones J Chem. 2015;15(2):179–86.
20 Periasamy S, Saravanan, KP. Aggregatibacter antinomycetemcomitans builds mutualistic biofilm communities with Fusobacterium nucleatum and Veillonella species in saliva. Infect Immun. 2009;77(9):3542–3551.
21. Shao H, Lamont R DD. Autoinducer 2 is Required for biofilm growth of Aggregatibacter actinomycetemcomitans. Infect Immun. 2007;75(9):4211–4218.
22. Novak E, Shao H, Daep C DD. Autoinducer-2 and QseC control biofilm formation and in vivo virulence of Aggregatibacter actinomycetemcomitans. Infect Immun. 2010;78(7):2919–26.
23. Widyarman AS, Suhalim OP, Nandary D, Theodorea CF. Pomegranate juice inhibits periodontal pathogens biofilm in vitro. Sci Dent J. 2018;2(3):101-108.
24. Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Curr Med Chem. 2015;22(1):132-49.
25. Cushnie TPT, Lamb AJ. Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents. 2011;38(2):99–107.
26. Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol. 2012;23(2):174-81.
27. Takasaki K, Fujise O, Miura M, Hamachi T, Maeda K. Porphyromonas gingivalis displays a competitive advantage over Aggregatibacter actinomycetemcomitans in co-cultured biofilm. J Periodontal Res. 2013;48(3):286–92.
28. Suryani L, Stepriyani S. Daya antibakteri infusa daun mahkota dewa (Phaleria macrocarpa) terhadap Staphylococcus aureus dan Eschericia coli. Mutiara Medika (Jurnal Kedokteran dan Kesehatan Universitas Muhammadiyah Yogyakarta). 2007;7(1):23–24.
29. Mutschler E, Widianto MB, Ranti AS, Padmawinata K, editors. Dinamika obat: buku ajar farmakologi dan toksikologi. Bandung: ITB; 1991. pp. 664-667.