Effect of Ethanol Extracts from Persea americana Leaves on HSC-3 Proliferation

  • Svetlana Tessalonica Undergraduate Student, Faculty of Dentistry, Trisakti University
  • Moehamad Orliando Roeslan Department of Histology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia


Introduction: Oral cancer has one of the highest death rates of all cancer types due to lack of treatment. Persea americana leaves consist of phenolic compounds that are known to have anticancer effects to inhibit the proliferation of human squamous carcinoma (HSC-3) cell lines. Ethanol extracts from P. americana leaves can inhibit the cell cycle in the G1/S and G2/M phases to give the cells time to repair damaged DNA. Objective: The aim of this study was to investigate the effect of ethanol extracts from P. americana leaves on HSC-3 proliferation. Methods: This study was conducted using experimental research and in vitro laboratory testing. Groups of extract consisted of four concentrations (100%, 50%, 25%, and 10%). Cytotoxic activity of the extracts was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium (MTT) assay. Result: P. americana leave ethanol extracts with concentrations of 100% had a significant difference (p <0.05) compared to negative control. Conclusion: Ethanol extracts from P. americana leaves could inhibit HSC-3 proliferation.


Widiyastuti Y, Pratiwi R, Riyanto S, Wahyuono S. Cytotoxic activity and apoptosis induction of avocado Persea americana Mill. seed extract on MCF-7 cancer cell line. Indones J Biotechnol. 2018;23(2):61-7.
Al-Dimassi S, Abou-Antoun T, El-Sibai M. Cancer cell resistance mechanism: a mini review. Clin Transl Oncol. 2014;16(6):511-6
Sarode GS, Sarode SC, Maniyar N, Anand R, Patil S. Oral cancer databases: A comprehensive review. J Oral Pathol Med. 2018;47(6):547-56.
Danaraddi S, Koneru A, Hunasgi S, Ramalu S. Natural ways to prevent and treat oral cancer. J Oral Res Rev. 2014;6(1):34-9.
Yasir M, Das S, Kharya MD. The phytochemical and pharmacological profile of Persea americana Mill. Pharmacogn Rev. 2010;4(7):77-84.
Bonilla-Porras AR, Salazar-Ospina A, Jimenez-Del-Rio M, Pereanez-Jimenez A, Velez-Pardo C. Pro-apoptotic effect of Persea americana var. Hass (avocado) on Jurkat lymphoblastic leukemia cells. Pharm Biol. 2013.
Mardiyaningsih A, Ismiyati N. Cytotoxic activity of ethanolic extract of Persea americana Mill. leaves on HeLa cervical cancer cell. Trad Med J. 2014;19(1):24-8.
Falodun A, Iyamabo H, Odion EE, Engel-Lutz N. Antiproliferative and Pro-apoptotic activities of the stem bark of Persea Americana (Lauraceae) mill in Human Breast Adenocarcinoma Cell Line. J Appl Sci Environ Manage. 2013;17 (3):433-8.
Khoswanto C, Juliastuti WS, Adla KA. The effect of Avocado leaf extract (Persea americana Mill.) on the fibroblast cells of post-extraction dental sockets in Wistar rats. Dent J. 2018;51(3):129-32.
Kemit N, Widarta IWR, Nocianitri KA. Pengaruh jenis pelarut dan waktu maserasi terhadap kandungan senyawa flavonoid dan aktivitas antioksidan ekstrak daun alpukat (Persea Americana Mill). 2016;5 (2):130-41.
Riss TL, Moravec RA, Niles AL, Duellman S, Benink HA, Worzella TJ, et al. Cell Viability Assays. In: Markossian S, Sittampalam GS, Grossman A, Brimacombe K, Arkin M, Auld D, et al., editors. Assay Guidance Manual. Bethesda (MD)2004.
Medawati A. Karsinoma sel skuamosa sebagai salah satu kanker rongga mulut dan permasalahannya. Insisiva Dent J. 2013;2 (1):87-90.
Nafis FDR, Sofian FF. Review jurnal: Aktivitas anti kanker payudara beberapa tanaman herbal. Farmaka. 2018;16 (2):84-95.
Mukhriani. Ekstraksi, pemisahan senyawa, dan identifikasi senyawa aktif. Jurnal Kesehatan. 2014;7 (2):361-67.
van Tonder A, Joubert AM, Cromarty AD. Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays. BMC Res Notes. 2015;8:47.
Falodun A, Engel N, Kragl U, Nebe B, Langer P. Novel anticancer alkene lactone from Persea americana. Pharm Biol. 2013;51(6):700-6.
Khalifa NS, Barakat HS, Elhallouty S, Salem D. Effect of the Water Extracts of Avocado Fruit and Cherimoya Leaf on Four Human Cancer Cell Lines and Vicia Faba Root Tip Cells. J Agric Sci. 2013;5(7):245-54.
Castro-Lopez C, Bautista-Hernandez I, Gonzalez-Hernandez MD, Martinez-Avila GCG, Rojas R, Gutierrez-Diez A, et al. Polyphenolic Profile and Antioxidant Activity of Leaf Purified Hydroalcoholic Extracts from Seven Mexican Persea americana Cultivars. Molecules. 2019;24(1).
Park S, Nam YH, Rodriguez I, Park JH, Kwak HJ, Oh Y, et al. Chemical constituents of leaves of Persea americana (avocado) and their protective effects against neomycin-induced hair cell damage. Revista Brasilera de Farmacognisa. 2019;29:739-43.
Putri EPK, Hamzah B, Rahman N. Analisis kualitatif zat bioaktif pada ekstrak daun alpukat (Persea americana Mill.) dan uji praklinis dalam menurunkan kadar glukosa darah pada mencit (mus musculus). J Akad Kim. 2013;2 (3):119-27.
Redha A. Flavonoid: Struktur, Sifat Antioksidatif Dan Peranannya Dalam Sistem Biologis. Jurnal Belian. 2010;9 (2):196-202.
Jeane M, Asih IARA, Bogoriani NW. Asupan Glikosida Flavonoid Terong Belanda (Solanum betaceum Cav.) Terhadap Aktivitas Superoksida Dismutase Dan Kadar Malondialdehid Tikus Wistar Yang Diberi Aktivitas Fisik Maksimal. Jurnal Media Sains 2018;2(1):32-6.
Ren W, Qiao Z, Wang H, Zhu L, Zhang L. Flavonoids: promising anticancer agents. Med Res Rev. 2003;23(4):519-34.
Salvador JA, Carvalho JF, Neves MA, Silvestre SM, Leitao AJ, Silva MM, et al. Anticancer steroids: linking natural and semi-synthetic compounds. Nat Prod Rep. 2013;30(2):324-74.
Yildrim I, Kutlu T. Anticancer Agents: Saponin and Tannin. Int J Biol Chem. 2015;9 (6):332-40.
Gonzalez-Sarrias A, Yuan T, Seeram NP. Cytotoxicity and structure activity relationship studies of maplexins A-I, gallotannins from red maple (Acer rubrum). Food Chem Toxicol. 2012;50(5):1369-76.
Barrajon-Catalan E, Fernandez-Arroyo S, Saura D, Guillen E, Fernandez-Gutierrez A, Segura-Carretero A, et al. Cistaceae aqueous extracts containing ellagitannins show antioxidant and antimicrobial capacity, and cytotoxic activity against human cancer cells. Food Chem Toxicol. 2010;48(8-9):2273-82.
Yang LL, Lee CY, Yen KY. Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells. Cancer Lett. 2000;157(1):65-75.
Tikoo K, Sane MS, Gupta C. Tannic acid ameliorates doxorubicin-induced cardiotoxicity and potentiates its anti-cancer activity: potential role of tannins in cancer chemotherapy. Toxicol Appl Pharmacol. 2011;251(3):191-200.
Bawadi HA, Bansode RR, Trappey A, 2nd, Truax RE, Losso JN. Inhibition of Caco-2 colon, MCF-7 and Hs578T breast, and DU 145 prostatic cancer cell proliferation by water-soluble black bean condensed tannins. Cancer Lett. 2005;218(2):153-62.
Larrosa M, Tomas-Barberan FA, Espin JC. The dietary hydrolysable tannin punicalagin releases ellagic acid that induces apoptosis in human colon adenocarcinoma Caco-2 cells by using the mitochondrial pathway. J Nutr Biochem. 2006;17(9):611-25.
Verrax J, Delvaux M, Beghein N, Taper H, Gallez B, Buc Calderon P. Enhancement of quinone redox cycling by ascorbate induces a caspase-3 independent cell death in human leukaemia cells. An in vitro comparative study. Free Radic Res. 2005;39(6):649-57.
Orrenius S, Gogvadze V, Zhivotovsky B. Mitochondrial oxidative stress: implications for cell death. Annu Rev Pharmacol Toxicol. 2007;47:143-83.
Barshteyn N, Elfarra AA. Formation of mono- and bis-Michael adducts by the reaction of nucleophilic amino acids with hydroxymethylvinyl ketone, a reactive metabolite of 1,3-butadiene. Chem Res Toxicol. 2009;22(5):918-25.
Briggs MK, Desavis E, Mazzer PA, Sunoj RB, Hatcher SA, Hadad CM, et al. A new approach to evaluating the extent of Michael adduct formation to PAH quinones: tetramethylammonium hydroxide (TMAH) thermochemolysis with GC/MS. Chem Res Toxicol. 2003;16(11):1484-92.
How to Cite
TESSALONICA, Svetlana; ROESLAN, Moehamad Orliando. Effect of Ethanol Extracts from Persea americana Leaves on HSC-3 Proliferation. Journal of Indonesian Dental Association, [S.l.], v. 3, n. 2, p. 65-70, oct. 2020. ISSN 2621-6175. Available at: <http://jurnal.pdgi.or.id/index.php/jida/article/view/464>. Date accessed: 02 dec. 2020. doi: https://doi.org/10.32793/jida.v3i2.464.
Research Article