The Effect of Curcuma zedoaria Extract on Enterococcus faecalis
Abstract
Introduction: Enterococcus faecalis is a microorganism that is commonly found in persistent or secondary infection of root canal treatment. Irrigation is one of the main processes of endodontic triad to eliminate microorganisms in root canal infections. Curcuma zedoaria, a medicinal plant with antibacterial properties, is effective against several types of microorganisms and has the potential to be used as an alternative of chemical-based irrigant. Objective: This study aimed to evaluate the antibacterial activity of Curcuma zedoaria extract against Enterococcus faecalis. Methods: This in vitro study used C. zedoaria extract with six concentrations (100%, 90%, 80%, 70%, 60%, 50%) as tested groups, 2% Chlorhexidine and distilled water were used as a positive and negative control group, respectively. Solid Mueller-Hinton Agar (MHA) medium containing Enterococcus faecalis ATCC 29212 was perforated with a cork borer and dropped by 5 mL solution according to each group. The inhibition zone diameter was measured to evaluate antibacterial activity. Data were analyzed using a One Way ANOVA, continued by a Post-hoc Bonferroni. Results: The largest growth inhibition zone was associated with the highest concentration (100%), with a mean diameter of 8.36 mm. Conclusion: The 100% C. zedoaria extract concentration had the most effective antibacterial potency against E. faecalis.
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Lobo R, Prabhu KS, Shirwaikar A, Shirwaikar A. Curcuma zedoaria Rosc. (white turmeric): a review of its chemical, pharmacological and ethnomedicinal properties. J Pharm Pharmacol. England; 2009 Jan;61(1):13–21.
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Malipeddi H, Ranjitha A, MV Ramana D, Anand M, Karigar A. Phytochemical screening and antioxidant activity of rhizome part Curcuma zedoaria. Int J Res Ayurveda Pharm. 2010 Dec 1;1:414–7.
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Rotstein I, Ingle JI. Ingle’s Endodontics. 7th ed. 2019.
Teles A, Manso MC, Loureiro S, Pina C, Cabeda J. Microorganisms: the reason to perform Endodontics. 2013. p. 1778–86.
Ruddle CJ. Endodontic triad for success: The role of minimally invasive technology. Dent Today. 2015;34(5):1–7.
Haapasalo M, Shen Y, Wang Z, Gao Y. Irrigation in endodontics. Br Dent J. 2014;216(6):299–303.
Schäfer E. Irrigation of the root canal. ENDO. 2007 Jan 1;1:11–27.
Sen BH, Türk T. An update on chlorhexidine in endodontics. Endod Prac. 2009 Jan 3;3(2):87–99.
Gomes BPFA, Vianna ME, Zaia AA, Almeida JFA, Souza-Filho FJ, Ferraz CCR. Chlorhexidine in endodontics. Braz Dent J. Brazil; 2013;24(2):89–102.
Kim J-W. Precipitate from a combination of sodium hypochlorite and chlorhexidine. Restor Dent Endod. Korea (South); 2012 Aug;37(3):185–6.
Haapasalo M, Shen Y, Qian W, Gao Y. Irrigation in endodontics. Dent Clin North Am. United States; 2010 Apr;54(2):291–312.
Das K, Rahman MA. Analgesic and Antimicrobial Activities of Curcuma zedoaria. Int J Pharm Pharm Sci. 2012;4(5):322–8.
Banisalam B, Sani W, Philip K, Imdadul H, Khorasani A. Comparison between in vitro and in vivo antibacterial activity of Curcuma zedoaria from Malaysia. African J Biotechnol. 2011 Sep 21;10:11676–81.
Chachad D. Antimicrobial Activity of Rhizomes of Curcuma zedoaria Rosc. 2015 Jan 1;6–391.
Zhang Q-W, Lin L-G, Ye W-C. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13:20.
Fahruddin AM, Tatengkeng F, Thamrin R, Riewpassa IE. Efektivitas Antibakteri Ekstrak Buah Patikala (Etlingera elatior (Jack) R.M.S.m) terhadap Bakteri Enterococcus faecalis. Dent J. 2016;5(3):69–75.
Lissanawidya P, Risandiansyah R, Airlangga H. Frekuensi Resistensi pada Enterococcus faecalis Terhadap Dekokta Rosella (Hibiscus sabdariffa Linn) dan Antibiotik Amoksisilin. J Islam Med Res. 2017;1(1):21–8.
Davis WW, Stout TR. Disc plate method of microbiological antibiotic assay. I. Factors influencing variability and error. Appl Microbiol. 1971 Oct;22(4):659–65.
Lobo R, Prabhu KS, Shirwaikar A, Shirwaikar A. Curcuma zedoaria Rosc. (white turmeric): a review of its chemical, pharmacological and ethnomedicinal properties. J Pharm Pharmacol. England; 2009 Jan;61(1):13–21.
Warbung Y. Daya Hambat Ekstrak Spons Laut Callyspongia sp terhadap Pertumbuhan Bakteri Staphylococcus aureus. e-GIGI. 2013 Nov 12;1(2):1–12.
Malipeddi H, Ranjitha A, MV Ramana D, Anand M, Karigar A. Phytochemical screening and antioxidant activity of rhizome part Curcuma zedoaria. Int J Res Ayurveda Pharm. 2010 Dec 1;1:414–7.
Ulpiyah Z, Shita ADP, Wahyukundari MA. Inhibition of namnam (Cynometra cauliflora L.) leaves extract on the growth of Porphyromonas gingivalis. Padjadjaran J Dent. 2019;31(2):106–11.
Published
2020-10-30
How to Cite
ANASTASIA, Danica; DESMARANI, Aina; BELLINDA, Merryca.
The Effect of Curcuma zedoaria Extract on Enterococcus faecalis.
Journal of Indonesian Dental Association, [S.l.], v. 3, n. 2, p. 61-64, oct. 2020.
ISSN 2621-6175.
Available at: <http://jurnal.pdgi.or.id/index.php/jida/article/view/498>. Date accessed: 22 dec. 2024.
doi: https://doi.org/10.32793/jida.v3i2.498.
Section
Research Article
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
