Perbedaan jumlah mikronukleus mukosa gingiva dan mukosa bukal akibat radiasi radiografi panoramik
Abstract
Objectives: Panoramic radiography exposure causes DNA damage and micronucleus formation. The gingival mucosa and buccal mucosa were used to identify the number of micronucleus due to radiation exposure because they have a high prevalence of oral cancer in Southeast Asia. This research is aimed to determine the difference between micronucleus formation at the buccal mucosa and the gingival mucosa after exposed by conventional panoramic radiography in the Dentomaxillofacial Radiology Installation of Prof. Soedomo dental hospital, Universitas Gadjah Mada.Material and Methods: Samples were obtained by rolling the cervical brush against the buccal and gingival mucosa at 10 days after radiation exposure. Samples were stained using the Feulgen-Rossenbeck method and analyzed under binuclear light microscope with a 400x magnification.
Results: Analysis of independent T tests showed that there was a significant difference (p<0,05) in the increasing of micronucleus formation between the buccal mucosa and the gingival mucosa. The average difference in the number of micronucleus were 5,5/1000 cells.
Conclusion: There were differences in the increasing of micronucleus between the buccal mucosa and the gingival mucosa due to exposure of conventional panoramic radiography. The buccal mucosa had higher increase than the gingival mucosa.
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Sandhu M, Mohan V, Kumar JS. Evaluation of genotoxic effect of X-rays on oral mucosa during panoramic radiography. J Indian Acad Oral Med Radiol. 2015;27(1):25–8.
Shantiningsih RR, Diba SF. Biological changes after dental panoramic exposure : conventional versus digital. Dent J. 2018;51(1):25–8.
Hiswara E. Buku Pintar Proteksi dan Keselamatan Radiasi di Rumah Sakit. Jakarta: Batan Press; 2015. 26–28 p.
Russo P. Handbook of X-ray Imaging Physics and Technology. Boca Raton: CRC Press; 2018. 425–431, 450 p.
White SC, Pharoah MJ. Oral Radiology Principles And Interpretation. 7th ed. St. Louis Missouri: Elseveier Mosby; 2014. 1,17,32,166.
Farman AG. Panoramic Radiology Seminars on Maxillofacial Imaging and Interpretation. Berlin: Springer; 2007. 29 p.
Visser, H., Hermann, K.P., Bredemeier, S., Kohler B. Comparison of Dosimetry in Conventional and Digital Panoramic Radiography. Mund Kiefer GesichtsChir. 2000;2000(4):213–6.
Shantiningsih RR, Suwaldi, Astuti I, Mudjosemedi M. Korelasi antara jumlah mikronukleus dan ekspresi 8-oxo-dG akibat paparan radiografi panoramic ( The correlation of micronucleus formation and 8-oxo-dG expression due to the panoramic radiography exposure ). Dent J. 2013;46(3):119–23.
McIntosh JR. Mechanism of Mitosis Chromosom Segregation. Basel: MDPI; 2017. 306–307 p.
Goncharuk VV. Drinking Water Physics, Chemistry and Biology. Switzerland: Springer; 2014. 364–365 p.
Shantiningsih RR. The Number of Micronucleus Between Single and Repeated X-rays Exposure of Panoramic Radiography Patients. 2nd Int Jt Symp Oral Dent Sci. 2012;1–5.
Shantiningsih, R.R., Diba, S.F., Awinda, A. & Rozaq AI. Increasing The Number of Micronucleus from Dental Radiation Effect Until 14th Day After Exposure. Int Symp Oral Dent Sci. 2013;1–9.
Torres-bugarín O, Romero NM, Luisa M, Ibarra R, Flores-garcía A, Aburto PV, et al. Genotoxic Effect in Autoimmune Diseases Evaluated by the Micronucleus Test Assay : Our Experience and Literature Review. J Biomed Biotechnol. 2015;2015(4):1–11.
Astbury C. Clinical Cytogenetics Clinics in Laboratory Medicine. Philadelphia: Saunders; 2011. 499 p.
Bergmeier LA. Oral Mucosa in Health and Disease: A Concise Handbook. London: Springer; 2018. 6–7 p.
Berkovitz, B.K.B., Holand, G.R., & Moxha BJ. Oral Anatomy, Histology and Embriology. 5th editio. China: Elsevier; 2018. 273–275 p.
Fehrenbach MJ, Popowics T. Ilustrated Dental Embryology, Histology, and Anatomy. 4th ed. Missouri: Elsevier; 2016. 120 p.
Singh, M. & Salnikova M. Novel Approaches and Strategies for Biologics, Vaccine and Cancer Therapies. waltham: Elsevier; 2015. 95 p.
Kesidi S, Maloth KN, Vinay K, Reddy K, Geetha P. Genotoxic and cytotoxic biomonitoring in patients exposed to full mouth radiographs – A radiological and cytological study. J Oral Maxillofac Radiol. 2017;5(1):1–6.
Cerqueira EM., Meireles JR., V.C. Junqueira, Gomes-Filho IS, Trindade S, Machado-Santelli GM. Genotoxic Effects of X-rays on Keratinized Mucosa Cells During Panoramic Dental Radiography. Dentomaxillofacial Radiol. 2008;37(7):398–403.
Waingade M, Medikeri RS. Analysis of micronuclei in buccal epithelial cells in patients subjected to panoramic radiography. Indian J Dent Res. 2012;23(5):574–8.
Kurniawati L. Kalibrasi Spasial Citra Radiografi dan Kalibrasi Dosis Mesin Sinar-X Panoramik Gigi. UGM; 2013.
Arora P, Devi P, Wazir SS. Evaluation of Genotoxicity in Patients Subjected to Panoramic Radiography by Micronucleus Assay on Epithelial Cells of the Oral Mucosa. J Dent Tehran Univ Med Sci. 2014;11(1):1–9.
Sandhu M, Mohan V, Kumar JS. Evaluation of genotoxic effect of X-rays on oral mucosa during panoramic radiography. J Indian Acad Oral Med Radiol. 2015;27(1):25–8.
Shantiningsih RR, Diba SF. Biological changes after dental panoramic exposure : conventional versus digital. Dent J. 2018;51(1):25–8.
Hiswara E. Buku Pintar Proteksi dan Keselamatan Radiasi di Rumah Sakit. Jakarta: Batan Press; 2015. 26–28 p.
Published
2020-05-10
How to Cite
SYARIFAH, Mufida Dzuriyatin; WIDYANINGRUM, Rini; SHANTININGSIH, Rurie Ratna.
Perbedaan jumlah mikronukleus mukosa gingiva dan mukosa bukal akibat radiasi radiografi panoramik.
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI), [S.l.], v. 4, n. 1, p. 11-15, may 2020.
ISSN 2686-1321.
Available at: <http://jurnal.pdgi.or.id/index.php/jrdi/article/view/424>. Date accessed: 23 nov. 2024.
doi: https://doi.org/10.32793/jrdi.v4i1.424.
Section
Original Research Article
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
