Perbedaan peningkatan jumlah mikronukleus antara mukosa gingiva dan mukosa bukal akibat paparan radiografi panoramik digital
Abstract
Objectives: The purpose of this study was to determine the differences in the number of micronuclei between the gingival mucosa and the buccal mucosa due to exposure to digital panoramic radiography.Material and Methods: This study used 10 healthy individuals aged 18-25 years. Exfoliated epithelial cell samples from the gingival and buccal mucosa were taken from the patient on the 10th day after exposure to digital panoramic radiography. The coloring method used is Feulgen-Rossenbeck.
Results: The micronucleus calculation was performed by the method of assessing 1000 cells per slide using a binuclear light microscope with a magnification of 400x. The results of the analysis by unpaired T test showed that the difference in the increase in the number of micronuclei was statistically significant (p <0.05). The average difference in the increase in the number of micronuclei was 3.5 ± 0.767. There is a significant difference in the increase in the number of micronuclei between the gingival mucosa and the buccal mucosa due to exposure to digital panoramic radiography with an increase in the number of micronucleus in the buccal mucosa higher than the gingival mucosa.
Conclusion: The conclusion of this study is that digital panoramic radiography can cause genotoxic effects on the gingival and buccal mucosa. Digital panoramic radiography is used if needed.
References
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Arora P, Devi P, Wazir SS. Evaluation of Genotoxicity in Patients Subjected to Panoramic Radiography by Micronucleus Assay on Epithelial Cells of the Oral Mucosa. Journal of Dentistry Tehran University of Medical Sciences. 2014;11(1): 1–9.
Cerqueira EM, Meireles JRC, Lopes MA, Junqueira VC, Gomes-Filho IS, Trindade S, Machado-Santelli GM. Genotoxic Effects of X-rays on Keratinized Mucosa Cells During Panoramic Dental Radiography. Dentomaxillofacial Radiology. 2008;37(7): 398–403.
Fehrenbach MJ, Popowics T. 2016. Ilustrated Dental Embryology, Histology, and Anatomy. 4th ed. Missouri: Elsevier; 2016. p.120.
Gavala S, Donta C, Tsiklakis K, Boziari A, Kamenopoulou V, Stamatakis HC. Radiation dose reduction in direct digital panoramic radiography. European Journal of Radiology. 2009;71(1): 42–48.
Kesidi S, Maloth KN, Reddy KVK, Geetha P. Genotoxic and cytotoxic biomonitoring in patients exposed to full mouth radiographs – A radiological and cytological study. Journal of Oral and Maxillofacial Radiology. 2017;5(1): 1–6.
Luzhna L, Kathiria P, Kovalchuk O. Micronuclei in genotoxicity assessment : from genetics to epigenetics and beyond. Frontiers In Genetics. 2013; 4(131): 1–17.
Madhavan R, Kumaraswamy M, Kailasam S, Kumar SM. Genetic Damage in Exfoliated Cells from Oral Mucosa of Individuals Exposed to X-rays after Panoramic Radiograph : A Cross-sectional Study. Journal of Indian Academy of Oral Medicine and Radiology. 2012;24(2): 102–105.
Nabilah I. Pengaruh Paparan Radiografi Panoramik Digital Tipe Langsung terhadap Peningkatan Jumlah Mikronukleus Mukosa Gingiva Manusia (Kajian di Instalasi Radiologi RSGM UGM Prof. Soedomo) [skripsi]. Yogyakarta: Fakultas Kedokteran Gigi UGM; 2018.
Naderi NJ, Farhadi S, Sarshar S. Micronucleus assay of buccal mucosa cells in smokers with the history of smoking less and more than 10 years Full Text Introduction. Indian Journal of Pathology and Microbiology. 2012;4(55): 433–438.
Pai A, Sharma RC, Naik RM, Guruprasad Y. Biomonitoring of genotoxic and cytotoxic effects of gingival epithelial cells exposed to digital panoramic radiography. Journal of Orofacial Science. 2012;4(2): 124–128.
Preethi N, Chikkanarasaiah N, Bethur SS. Genotoxic effects of X-rays in buccal mucosal cells in children subjected to dental radiographs. BDJ Open. 2016. Vol 2. Pp: 1–6.
Sabharwal R, Verma P, Syed MA, Sharma T, Subudhi SK, Mohanty S, Gupta S. Emergence of micronuclei as a genomic biomarker. Indian J Med Paediatr Oncol. 2015;36(4): 212–218.
Sandhu M, Mohan V, Kumar JS. Evaluation of genotoxic effect of X-rays on oral mucosa during panoramic radiography. Journal of Indian Academy of Oral Medicine and Radiology. 2015;27(1): 25–28.
Shantiningsih RR, Diba SF, Awinda A, Rozaq AI. 2013. Increasing The Number Of Micronucleus from Dental Radiation Effect Until 14th Day After Exposure, Proceedings of The International Symposium on Oral and Dental Science; 2013 Jan 17-18; Yogyakarta: Gadjah Mada University; 2013.
Shantiningsih RR, Suwaldi, Astuti I, Mudjosemedi M. Korelasi antara jumlah mikronukleus dan ekspresi 8-oxo-dG akibat paparan radiografi panoramic (The correlation of micronucleus formation and 8-oxo-dG expression due to the panoramic radiography exposure). Dental Journal. 2013b;46(3): 119–123.
Sivasankari P, Kaur S, Reddy, Vivekanandam, Rao R. Micronucleus Index : An Early Diagnosis In Oral Carcinoma Micronucleus Index : An Early Diagnosis In Oral Carcinoma. J. Anat. Soc. India. 2016; 57(1): 8–13.
Waingade M, Medikeri RS. Analysis of micronuclei in buccal epithelial cells in patients subjected to panoramic radiography. Indian Journal of Dental Research. 2012;23(5): 574–578.
Whaites E, Drage N. Essential of Dental Radiography and Radiology. 5th ed. Toronto: Elsevier; 2013. p. 3, 15, 16, 61, 79,180.
White SC, Pharoah MJ. Oral Radiology Principles And Interpretation. 7th ed. St. Louis Missouri: Elseveier Mosby; 2013. p. 1, 9, 10, 11, 17, 32,166.
Published
2019-08-30
How to Cite
OLVYA, Shintia; SURYANI, Isti Rahayu; SHANTININGSIH, Rurie Ratna.
Perbedaan peningkatan jumlah mikronukleus antara mukosa gingiva dan mukosa bukal akibat paparan radiografi panoramik digital.
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI), [S.l.], v. 3, n. 2, p. 1-6, aug. 2019.
ISSN 2686-1321.
Available at: <http://jurnal.pdgi.or.id/index.php/jrdi/article/view/483>. Date accessed: 26 apr. 2024.
doi: https://doi.org/10.32793/jrdi.v3i2.483.
Section
Original Research Article
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
